Studies Find Quercetin & Natto K2 INHIBIT P-Glycoprotein; This Drastically Increases Ivermectin Toxicity!

A War On P-Glycoprotein? Depopulation binary weapons

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We’ve discussed the role of P-glycoprotein in reducing ivermectin toxicity by limiting the toxic pesticide’s exposure to our brains and testes (among other vital tissues). In that article we also covered how ivermectin, hydroxychloriquine & some statins are P-glycoprotein inhibitors, which means they weaken the Pgp defenses against ivermectin at some very important tissues.

Today, I want to cover two more Pgp inhibitors: quercetin & natto, which have been pushed by some people who also promote ivermectin.

Natto K2 Inhibits Caco-2 Cells’ Pgp & Digoxin Flux

In a paper called ‘Inhibition of P-glycoprotein in Caco-2 cells: effects of herbal remedies frequently used by cancer patients’ by Engdal and Nilsen, the researchers examined Natto K2’s inhibition of digoxin flux for Caco-2 human colorectal adenocarcinoma cells line (full report). Natto K2 is a type of “Vitamin K2” found in many “Vitamin K2” supplements as well as included in various nattokinase products (unless it is removed entirely from the natto) Digoxin is a Pgp substrate.

“This study shows that … Natto K2 … inhibit[s] P-gp in vitro.”

“Special attention should be paid .. to Natto K2 due to low IC50 value and IC50/IC25 ratio”

Quercetin Vs Pgp And Rh123 Influx In KB-V1 Human Cancer Cell Line

In a 2005 paper called ‘Inhibition of P-glycoprotein function and expression by kaempferol and quercetin’ by Limtrakul et al researchers examined quercetin’s effect on the influx of the Pgp substrate Rh123 in the KB-V1 human cancer cell line. (full report)

“The Pgp level in KB-V1 cells were decreased markedly in the presence of 10 and 30 μM quercetin or kaempferol for a period of 48 h in a dose-dependent manner (Figure 4). Quercetin and kaempferol at a concentration of 30 μM significantly decreased (P<0.05) the Pgp expression in KB-V1 cells.”

Study Finds Quercetin Inhibits P-Glycoprotein In Hamster Ovary Cells

In a 1997 paper called ‘Effect of Quercetin on Hoechst 33342 Transport by Purified and
Reconstituted P-Glycoprotein’ by Shapiro and Ling, the researchers examined the effect of Quercetin on transport of the Pgp substrate Hoechst 33342 in hamster ovary cell lines (full report). Two hamster ovary cell lines were examined including the CHRC5 “multi drug resistant” cell line.

“Quercetin reduced the rate and extent of the decrease of Hoechst 33342 fluorescence of both cell lines”

“Our preliminary immunocytofluorimetry studies indicate that Quercetin reduces the amount of Pgp.”

“Treatment of the cells with 100 μM quercetin during Hoechst 33342 uptake resulted in a reduction of Hoechst 33342 fluorescence in Aux Bl cells… Increased Hoechst 33342 uptake by CHRC5 cells suggests a possible inhibition of P-glycoprotein by quercetin.”

“Quercetin inhibited ATP hydrolysis by P-glycoprotein”

“Quercetin inhibited the ATPase activity of P-glycoprotein, impairing its transport function.”

“Since drug transport by P-glycoprotein is dependent upon ATP hydrolysis [3, 6, 27, 281, we checked for inhibition of the ATPase activity of P-glycoprotein by quercetin. Figure 5 shows that, indeed, quercetin inhibits the ATPase activity of P-glycoprotein.”

Quercitin’s Inhibition Of P-glycoprotein In Wistar Rats & In Vitro

In a 2008 paper called ‘Pre-clinical evidence for altered absorption and biliary excretion of irinotecan (CPT-11) in combination with quercetin: Possible contribution of P-glycoprotein’ by Bansal et al, researchers examined a Pgp substrate called irinotecan (aka CPT-11). (full report)

“The concomitant administration of naturally occurring dietary supplement quercetin significantly inhibited the P-gp both in vitro and in vivo”

“The amounts of irinotecan and SN-38 eliminated in bile in control rats, is
reduced to almost half when treated with quercetin.”

Much more intact CPT-11 was excreted when administered IV compared to the oral levels. For the IV dosing, the researchers found “about 8–9% of irinotecan dose was recovered unchanged in bile in control rats while in treatment group it got reduced to 5%.”

“The increase of absorptive transport by 20 μM quercetin … in Caco-2 cells is comparable to 100 μM verapamil, indicating that quercetin is a fairly potent P-gp inhibitor”

“Based on the results of our in vitro studies in Caco-2 cells, quercetin
is a potent inhibitor of P-gp”

Study Finds Quercetin Reverses Multi Drug Resistance In MCF-7 Human Cell Line

In a 1994 paper called ‘Quercetin potentiates the effect of adriamycin in a multidrug-resistant MCF-7 human breast-cancer cell line: P-glycoprotein as a possible target’ by Scambia et al (full report) the researchers examined the Pgp substrate Rh123 and the increased levels of Rh123 as the Quercetin was increased. This apparently coincided with a lowered amount of immunoreactive Pgp.

“In a comparison of the curves of the cumulative frequencies of fluorescence intensities from the control and Q-treated cells (Fig. 5 B), it appeared that 10 μM lowered the amount of immunoreactive Pgp.”

“At present, our results do not explain the mechanism by which Q modulates Pgp activity and reverses MDR. A direct interaction between Q and Pgp may be possible”

Next Up:

Pig Study Raises MAJOR Questions Of Dangers Of Combining Quercetin & Ivermectin